Multiplexed Analysis of Serum Breast and Ovarian Cancer Markers by Means of Suspension Bead–quantum Dot Microarrays

Kristina Brazhnik (1) , Zinaida Sokolova (2) , Maria Baryshnikova (2) , Regina Bilan (1) , Igor Nabiev (3) , Alyona Sukhanova (3)

1 The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia]
2 N.N. Blokhin Russian Cancer Research Center
3 LRN - Laboratoire de Recherche en Nanosciences - EA 4682

Multiplexed analysis of cancer markers is crucial for early tumor diagnosis and screening. We have designed lab-on-a-bead microarray for quantitative detection of three breast cancer markers in human serum. Quantum dots were used as bead-bound fluorescent tags for identifying each marker by means of flow cytometry. Antigen-specific beads reliably detected CA 15-3, CEA, and CA 125 in serum samples, providing clear discrimination between the samples with respect to the antigen levels. The novel microarray is advantageous over the routine single-analyte ones due to the simultaneous detection of various markers. Therefore the developed microarray is a promising tool for serum tumor marker profiling.

Mots clés: quantum dots; cancer markers; lab-on-a-bead; multiplexed analysis; flow cytometry

Domaines: Micro et nanotechnologies/Microélectronique; Imagerie; Cancer

Kristina Brazhnik, Zinaida Sokolova, Maria Baryshnikova, Regina Bilan, Igor Nabiev, et al.. Multiplexed Analysis of Serum Breast and Ovarian Cancer Markers by Means of Suspension Bead–quantum Dot Microarrays. Physics Procedia, 2015, 73, pp.235 - 240. ⟨10.1016/j.phpro.2015.09.163⟩. ⟨hal-03112393⟩