Skip to Main content Skip to Navigation
Journal articles

Beta-nerve growth factor stimulates spontaneous electrical activity of in vitro embryonic mouse GnRH neurons through a P75 mediated-mechanism

Abstract : The control of ovulation helps guarantee the success of reproduction and as such, contributes to the fitness of a species. In mammals, two types of ovulation are observed: induced and spontaneous ovulation. Recent work on camelids, that are induced ovulators, highlighted the role of a factor present in seminal plasma, beta Nerve Growth Factor (β-NGF), as the factor that triggers ovulation in a GnRH dependent manner. In the present work, we characterized alpaca β-NGF (aβ-NGF) and its 3D structure and compared it with human recombinant β-NGF (hβ-NGF). We showed that the β-NGF enriched fraction of alpaca semen and the human recombinant protein, both stimulated spontaneous electrical activity of primary GnRH neurons derived from mouse embryonic olfactory placodes. This effect was dose-dependent and mediated by p75 receptor signaling. P75 receptors were found expressed in vitro by olfactory ensheathing cells (OEC) in close association with GnRH neurons and in vivo by tanycytes in close vicinity to GnRH fibers in adult mouse. Altogether, these results suggested that β-NGF induced ovulation through an increase in GnRH secretion provoked by a glial dependent P75 mediated mechanism.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03094626
Contributor : Anne Duittoz Connect in order to contact the contributor
Submitted on : Monday, January 4, 2021 - 1:25:38 PM
Last modification on : Thursday, November 25, 2021 - 11:20:03 AM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Links full text

Identifiers

Collections

Citation

Caroline Pinet-Charvet, Renaud Fleurot, Flavie Derouin-Tochon, Simon de Graaf, Xavier Druart, et al.. Beta-nerve growth factor stimulates spontaneous electrical activity of in vitro embryonic mouse GnRH neurons through a P75 mediated-mechanism. Scientific Reports, Nature Publishing Group, 2020, 10 (1), 14 p. ⟨10.1038/s41598-020-67665-4⟩. ⟨hal-03094626⟩

Share

Metrics

Record views

125