Characterizing single sinonasal squamous cell carcinoma using dielectrophoresis and electrorotation

Thao N P Mai (1) , Sakina Bensalem (1) , Bénédicte Thiebot (2) , Philippe Manivet (3) , Juan Pelta (2) , Bruno Le Pioufle (1)

1 IDA - Institut d'Alembert
2 LAMBE - UMR 8587 - Laboratoire Analyse, Modélisation et Matériaux pour la Biologie et l'Environnement
3 Hôpital Lariboisière-Fernand-Widal [APHP]

We show the capture and analysis single cells, in particular human sinonasal squamous cell carcinomas (SCC), by the combination of di-electrophoresis (DEP) force and electrorotation, within a microfluidic device. A set of 4 planar polynomial electrodes was employed to perform nDEP trapping of two lines tumor cell with different invasivity, named NC5 and NC7. Once captured at the center of the electrodes set, electrorotation was served to extract the rotational speed's spectra. From the spectra, their electrophysiological properties can be estimated.

Mots clés: Sinonasal squamous cell carcinomas; di-electrophoresis; electrorotation; single cell analysis

Domaines: Micro et nanotechnologies/Microélectronique; Sciences du Vivant [q-bio]

Thao N P Mai, Sakina Bensalem, Bénédicte Thiebot, Philippe Manivet, Juan Pelta, et al.. Characterizing single sinonasal squamous cell carcinoma using dielectrophoresis and electrorotation. MicroTAS, Oct 2020, on-line, France. ⟨hal-03078871⟩