A resource of targeted mutant mouse lines for 5,061 genes

Marie-Christine Birling (1) , Atsushi Yoshiki , David Adams , Shinya Ayabe , Arthur Beaudet , Joanna Bottomley , Allan Bradley , Steve Dm Brown , Antje Bürger , Wendy Bushell , Francesco Chiani , Hsian-Jean Genie Chin , Skevoulla Christou , Gemma Codner , Francesco Demayo , Mary Dickinson , Brendan Doe , Leah Rae Donahue , Martin Fray , Alessia Gambadoro , Xiang Gao , Marina Gertsenstein , Alba Gomez-Segura , Leslie Goodwin , Jason Heaney , Yann Hérault (1) , Martin Hrabe de Angelis , Si-Tse Jiang , Monica Justice , Petr Kasparek , Ruairidh King , Ralf Kühn , Ho Lee , Young Jae Lee , Zhiwei Liu , K Kent Lloyd , Isabel Lorenzo , Ann-Marie Mallon , Colin Mckerlie , Terrence Meehan , Stuart Newman , Lauryl Mj Nutter , Goo Taeg Oh , Guillaume Pavlovic (1) , Ramiro Ramirez-Solis , Barry Rosen , Edward Ryder , Luis Santos , Joel Schick , John Seavitt , Radislav Sedlacek , Claudia Seisenberger , Je Kyung Seong , William Skarnes , Tania Sorg (1) , Karen Steel , Masaru Tamura , Glauco Tocchini-Valentini , Chi-Kuang Leo Wang , Hannah Wardle-Jones , Marie Wattenhofer-Donzé (1) , Sara Wells , Brandon Willis , Joshua Wood , Wolfgang Wurst , Ying Xu , Lydia Teboul , Stephen Murray

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for over 5,000 genes from targeted embryonic stem cells on the C57BL/6N genetic background. This includes 2,850 null alleles for which no equivalent mutant mouse line exists, 2,987 novel conditional-ready alleles, and 4,433 novel reporter alleles. This nearly triples the number of genes with reporter alleles and almost doubles the number of conditional alleles available to the scientific community. When combined with more than 30 years of community effort, the total mutant allele mouse resource covers more than half of the genome. The extensively validated collection is archived and distributed through public repositories, facilitating availability to the worldwide biomedical research community, and expanding our understanding of gene function and human disease.

Domaines: Sciences du Vivant [q-bio]

Marie-Christine Birling, Atsushi Yoshiki, David Adams, Shinya Ayabe, Arthur Beaudet, et al.. A resource of targeted mutant mouse lines for 5,061 genes. Nature Genetics, 2021, ⟨10.1038/s41588-021-00825-y⟩. ⟨hal-03065452⟩