In Vitro Reconstitution of Dynein Force Exertion in a Bulk Viscous Medium

Héliciane Palenzuela (1) , Benjamin Lacroix (1) , Jérémy Sallé (1) , Katsuhiko Minami (2) , Tomohiro Shima (2) , Antoine Jegou (1) , Guillaume Romet ‐ Lemonne (1) , Nicolas Minc (1)

1 IJM (UMR_7592) - Institut Jacques Monod
2 Graduate School of Science, The University of Tokyo, 113-0033 Tokyo, Japan

Highlights d A bulk motility assay reconstitutes dynein pulling microtubules in the cytoplasm d A single motile cargo in a viscous solution generates near pN forces on a microtubule d Forces are additive, recapitulating a length-dependent model d Tug-of-war situations yield centration of minimal asters in bulk

Domaines: Sciences du Vivant [q-bio]; Biophysique; Biologie cellulaire; Biochimie, Biologie Moléculaire; Organisation et fonctions cellulaires [q-bio.SC]

Héliciane Palenzuela, Benjamin Lacroix, Jérémy Sallé, Katsuhiko Minami, Tomohiro Shima, et al.. In Vitro Reconstitution of Dynein Force Exertion in a Bulk Viscous Medium. Current Biology - CB, 2020, 30 (22), pp.4534-4540.e7. ⟨10.1016/j.cub.2020.08.078⟩. ⟨hal-03025707⟩