Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation

Fabiana Cerqueira Campos , Cynthia Dennis , Hervé Alégot , Cornelia Fritsch , Adam Isabella , Pierre Pouchin , Olivier Bardot , Sally Horne-Badovinac , Vincent Mirouse (1)

1 GReD - Génétique, Reproduction et Développement

How extracellular matrix contributes to tissue morphogenesis is still an open question. In the Drosophila ovarian follicle, it has been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils and stress fibers and their respective impact on elongation are unclear. We found that Dystroglycan (Dg) and Dystrophin (Dys) are involved in BM fibril deposition. Moreover, they also orient stress fibers, by acting locally and in parallel to Fat2. Importantly, Dg-Dys complexmediated cell-autonomous control of F-actin fiber orientation relies on the preceding BM fibril deposition, indicating two distinct but interdependent functions. Thus, the Dg-Dys complex works as a crucial organizer of the epithelial basal domain, regulating both F-actin and BM. Furthermore, BM fibrils act as a persistent cue for the orientation of stress fibers that are the main effector of elongation.

Mots clés: Morphogenesis; Extracellular matrix; Drosophila; Tissue elongation; Planar cell polarity; Dystrophin; Dystroglycan; Basement membrane

Domaines: Sciences du Vivant [q-bio]

Fabiana Cerqueira Campos, Cynthia Dennis, Hervé Alégot, Cornelia Fritsch, Adam Isabella, et al.. Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation. Development (Cambridge, England), 2020, 147 (7), pp.dev186957. ⟨10.1242/dev.186957⟩. ⟨hal-03009513⟩