Probiotic from human breast milk, Lactobacillus fermentum, promotes growth in animal model of chronic malnutrition

Pierre Poinsot (1) , Armelle Penhoat (2) , Mélanie Mitchell (1) , Valérie Sauvinet (3, 2) , Laure Meiller (2, 3) , Corinne Louche-Pélissier , E. Meugnier (2) , Mathias Ruiz (2, 4) , Martin Schwarzer (1) , Marie-Caroline Michalski (5, 2) , F. Leulier (1) , N. Peretti (2)

1 IGFL - Institut de Génomique Fonctionnelle de Lyon
2 CarMeN - Cardiovasculaire, métabolisme, diabétologie et nutrition
3 Centre de Recherche en Nutrition Humaine
4 HFME - Hôpital Femme Mère Enfant [CHU - HCL]
5 INRA - Institut National de la Recherche Agronomique

BACKGROUND: Chronic undernutrition leads to growth hormone resistance and poor growth in children, which has been shown to be modulated by microbiota. We studied whether Lactobacillus fermentum CECT5716 (Lf (CECT5716)), isolated from mother's breast milk, could promote juvenile growth through the modulation of lipid absorption in a model of starvation. METHODS: Germ-free (GF) Drosophila melanogaster larvae were inoculated with Lf (CECT5716) in conditions of undernutrition with and without infant formula. The impact of Lf (CECT5716) on larval growth was assessed 7 days after egg laying (AED) by measuring the larval size and on maturation by measuring the emergence of pupae during 21 days AED. For lipid absorption test, Caco2/TC7 intestinal cells were incubated with Lf (CECT5716) and challenged with mixed lipid micelles. RESULTS: The mono-associated larvae with Lf (CECT5716) were significantly longer than GF larvae (3.7 vs 2.5 mm; p \textless 0.0001). The effect was maintained when Lf (CECT5716) was added to the infant formula. The maturation time of larvae was accelerated by Lf (CECT5716) (12 vs 13.2 days; p = 0.01). Lf (CECT5716) did not have significant impact on lipid absorption in Caco2/TC7 cells. CONCLUSIONS: Lf (CECT5716) is a growth-promoting strain upon undernutrition in Drosophila, with a maintained effect when added to an infant formula but without effect on lipid absorption in vitro.

Domaines: Sciences du Vivant [q-bio]

Pierre Poinsot, Armelle Penhoat, Mélanie Mitchell, Valérie Sauvinet, Laure Meiller, et al.. Probiotic from human breast milk, Lactobacillus fermentum, promotes growth in animal model of chronic malnutrition. Pediatric Research, 2020, 88 (3), pp.374 - 381. ⟨10.1038/s41390-020-0774-0⟩. ⟨hal-02998911⟩