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Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila

Abstract : Monalysin was recently described as a novel pore-forming toxin (PFT) secreted by the Drosophila pathogen Pseudomonas entomophila. Recombinant monalysin is multimeric in solution, whereas PFTs are supposed to be monomeric until target membrane association. Monalysin crystals were obtained by the hanging-drop vapour-diffusion method using PEG 8000 as precipitant. Preliminary X-ray diffraction analysis revealed that monalysin crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 162.4, b = 146.2, c = 144.4 Å, [beta] = 122.8°, and diffracted to 2.85 Å resolution using synchrotron radiation. Patterson self-rotation analysis and Matthews coefficient calculation indicate that the asymmetric unit contains nine copies of monalysin. Heavy-atom derivative data were collected and a Ta6Br14 cluster derivative data set confirmed the presence of ninefold noncrystallographic symmetry.
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https://hal.archives-ouvertes.fr/hal-02989353
Contributor : Philippe Leone Connect in order to contact the contributor
Submitted on : Thursday, November 5, 2020 - 10:22:21 AM
Last modification on : Monday, January 31, 2022 - 5:30:06 PM

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Philippe Leone, Maryline Blemont, Renaud Vincentelli, Christine Kellenberger, Onya Opota, et al.. Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, International Union of Crystallography, 2013, 69 (8), pp.930-933. ⟨10.1107/S174430911301885X⟩. ⟨hal-02989353⟩

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