WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity

Emmanuelle Steib , Marine H. Laporte , Davide Gambarotto , Natacha Olieric , Celine Zheng , Susanne Borgers , Vincent Olieric , Maeva Le Guennec , France Koll (1, 2) , Anne-Marie Tassin (1, 2) , Michel O. Steinmetz , Paul Guichard , Virginie Hamel

1 I2BC - Institut de Biologie Intégrative de la Cellule
2 BIOCIL - Biogenèse et Fonction de la Structure Centriolaire et Ciliaire

Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying the stability of microtubule triplets, as well as centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy for nanoscale protein mapping, we reveal that POC16 and its human homolog WDR90 are components of the microtubule wall along the central core region of the centriole. We further found that WDR90 is an evolutionary microtubule associated protein. Finally, we demonstrate that WDR90 depletion impairs the localization of inner scaffold components, leading to centriole structural abnormalities in human cells. Altogether, this work highlights that WDR90 is an evolutionary conserved molecular player participating in centriole architecture integrity.

Mots clés: centrosome; chlamydomonas; cilia; delta-tubulin; expansion microscopy; inner scaffold; microtubule; reveals; basal bodies; cartwheel; cells; centriole

Domaines: Sciences du Vivant [q-bio]

Emmanuelle Steib, Marine H. Laporte, Davide Gambarotto, Natacha Olieric, Celine Zheng, et al.. WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. eLife, 2020, 9, pp.e57205. ⟨10.7554/eLife.57205⟩. ⟨hal-02968377⟩