Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates

Adama Sanou (1) , Zekiba Tarnagda (2) , Estelle Kanyala (3) , Dezemon Zingué (1) , Moumini Nouctara (1) , Zakaria Ganamé (1) , Adjima Combary , Hervé Hien (1, 2) , Mathurin Dembele , Antoinette Kabore (1) , Nicolas Meda (1) , Philippe van de Perre (4, 5) , Dorine Neveu (5) , Anne-Laure Bañuls (6) , Sylvain Godreuil (4, 5)

1 Centre Muraz [Bobo-Dioulasso, Burkina Faso]
2 IRSS - Institut de Recherche en Sciences de la Santé
3 UJZK - Université Joseph Ki-Zerbo [Université de Ouagadougou, Burkina Faso]
4 Département de Bactériologie-Virologie [CHRU Montpellier]
5 Pathogénèse et contrôle des infections chroniques (PCCI)
6 MIVEGEC-GeneSys - Du gène à l'écosystème

Background: In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations.Methodology/principal findings: Twenty six M. bovis strains were isolated from 101 cattle carcasses with suspected bTB lesions during routine meat inspections at the Bobo Dioulasso and Ouagadougou slaughterhouses. In addition, 7 M. bovis strains were isolated from 576 patients with pulmonary tuberculosis. Spoligotyping, RDAf1 deletion and MIRU-VNTR typing were used for strains genotyping. The isolation of M. bovis strains was confirmed by spoligotyping and 12 spoligotype signatures were detected. Together, the spoligotyping and MIRU-VNTR data allowed grouping the 33 M. bovis isolates in seven clusters including isolates exclusively from cattle (5) or humans (1) or from both (1). Moreover, these data (genetic analyses and phenetic tree) showed that the M. bovis isolates belonged to the African 1 (Af1) clonal complex (81.8%) and the putative African 5 (Af5) clonal complex (18.2%), in agreement with the results of RDAf1 deletion typing.Conclusions/significance: This is the first detailed molecular characterization of M. bovis strains from humans and cattle in Burkina Faso. The distribution of the two Af1 and putative Af5 clonal complexes is comparable to what has been reported in neighbouring countries. Furthermore, the strain genetic profiles suggest that M. bovis circulates across the borders and that the Burkina Faso strains originate from different countries, but have a country-specific evolution. The genetic characterization suggests that, currently, M. bovis transmission occurs mainly between cattle, occasionally between cattle and humans and potentially between humans. This study emphasizes the bTB risk in cattle but also in humans and the difficulty to set up proper disease control strategies in Burkina Faso.

Domaines: Santé publique et épidémiologie; Bactériologie; Médecine vétérinaire et santé animale; Maladies infectieuses

Adama Sanou, Zekiba Tarnagda, Estelle Kanyala, Dezemon Zingué, Moumini Nouctara, et al.. Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates. PLoS Neglected Tropical Diseases, 2014, 8 (10), pp.e3142. ⟨10.1371/journal.pntd.0003142⟩. ⟨hal-02967869⟩