Insecticide resistance status in Anopheles gambiae in southern Benin

Anges Yadouleton (1) , Gil Padonou (1) , Alex Asidi (2) , Nicolas Moiroux (3) , Sahabi Bio-Banganna (1) , Vincent Corbel (1, 4) , Raphael N'Guessan (1, 2) , Dina Gbenou (5) , Imorou Yacoubou , Kinde Gazard (6) , Martin Akogbeto (1)

1 CREC - Centre de Recherche Entomologique de Cotonou
2 LSHTM - London School of Hygiene and Tropical Medicine
3 MIVEGEC-VCG - Vector Control Group
4 Caractérisation et contrôle des populations de vecteurs
5 WHO, Country Office for Benin [Cotonou, Benin]
6 UAC - Université d’Abomey-Calavi = University of Abomey Calavi

Background : The emergence of pyrethroid resistance in Anopheles gambiaehas become a serious concern to thefuture success of malaria control. In Benin, the National Malaria Control Programme has recently planned to scaling up long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) for malaria prevention. It is, therefore,crucial to monitor the level and type of insecticide resistance in An. gambiae, particularly in southern Benin where reduced efficacy of insecticide-treated nets (ITNs) and IRS has previously been reported. Methods : The protocol was based on mosquito collection during both dry and rainy seasons across forty districts selected in southern Benin. Bioassay were performed on adults collected from the field to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, andbendiocarb 0.1%) following WHOPES guidelines. The species within An. gambiaecomplex, molecular form and presence of kdr and ace-1 mutations were determined by PCR. Results : Strong resistance to permethrin and DDT was found in An. gambiae populations from southern Benin, except in Aglangandan where mosquitoes were fully susceptible (mortality 100%) to all insecticides tested. PCR showed the presence of two sub-species of An. gambiae, namely An. gambiae s.s, and Anopheles melas, with a predominance for An. gambiae s.s(98%). The molecular M form of An. gambiaewas predominant in southern Benin (97%). The kdr mutation was detected in all districts at various frequency (1% to 95%) whereas theAce-1mutation was found at a very low frequency (≤5%). Conclusion : This study showed a widespread resistance to permethrin in An. gambiae populations from southern Benin, with a significant increase of kdr frequency compared to what was observed previously in Benin. The low frequency ofAce-1recorded in all populations is encouraging for the use of bendiocarb as an alternative insecticide to pyrethroids for IRS in Benin.

Domaines: Zoologie des invertébrés

Anges Yadouleton, Gil Padonou, Alex Asidi, Nicolas Moiroux, Sahabi Bio-Banganna, et al.. Insecticide resistance status in Anopheles gambiae in southern Benin. Malaria Journal, 2010, 9, pp.83. ⟨10.1186/1475-2875-9-83⟩. ⟨hal-02965709⟩