Surface-Directed Selection of Dynamic Constitutional Frameworks as an Optimized Microenvironment for Controlled Enzyme Activation

Yan Zhang (1) , Mihail Barboiu (2) , Olof Ramström (3) , Jinghua Chen (1)

1 Jiangnan University
2 IEM - Institut Européen des membranes
3 UMass Lowell - University of Massachusetts [Lowell]

Dynamic constitutional frameworks composed of cross-linked networks of imine-exchanging components have been generated and applied to the establishment of optimal microenvironments for carbonic anhydrase (CA) in aqueous solution. In response to the dynamic recomposition process, the enzyme showed distinct differential preferences for interchanging and incorporation of the amine functionalities, which were furthermore in good correlation with their inverse activation effects of CA. The results demonstrated surface-directed selection of the enzyme environment, where amines with lower enzyme-directed incorporation ratio possess higher activation effects, leading to a strategy of self-optimization of the enzyme microenvironment for better catalytic performances.

Domaines: Matériaux; Catalyse

Yan Zhang, Mihail Barboiu, Olof Ramström, Jinghua Chen. Surface-Directed Selection of Dynamic Constitutional Frameworks as an Optimized Microenvironment for Controlled Enzyme Activation. ACS Catalysis, 2019, 10 (2), pp.1423-1427. ⟨10.1021/acscatal.9b04938⟩. ⟨hal-02946339⟩