Skip to Main content Skip to Navigation
New interface
Journal articles

Multimodal Imaging Mass Spectrometry to Identify Markers of Pulmonary Arterial Hypertension in Human Lung Tissue using MALDI-ToF, ToF-SIMS and Hybrid SIMS

Abstract : Pulmonary arterial hypertension (PAH) is a rare and deadly disease affecting roughly 15–60 people per million in Europe with a poorly understood pathology. There are currently no diagnostic tools for early detection nor does a curative treatment exist. The lipid composition of arteries in lung tissue samples from human PAH and control patients were investigated using matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) combined with time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging. Using random forests as an IMS data analysis technique, it was possible to identify the ion at m/z 885.6 as a marker of PAH in human lung tissue. The m/z 885.6 ion intensity was shown to be significantly higher around diseased arteries and was confirmed to be a diacylglycerophosphoinositol PI(C18:0/C20:4) via MS/MS using a novel hybrid SIMS instrument. The discovery of a potential biomarker opens up new research avenues which may finally lead to a better understanding of the PAH pathology and highlights the vital role IMS can play in modern biomedical research.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02908467
Contributor : Alain Brunelle Connect in order to contact the contributor
Submitted on : Tuesday, October 5, 2021 - 10:05:29 AM
Last modification on : Wednesday, November 9, 2022 - 4:46:08 PM
Long-term archiving on: : Thursday, January 6, 2022 - 6:18:12 PM

File

AnalChem_2020_92_12079_VanNuff...
Files produced by the author(s)

Identifiers

Citation

Sebastiaan van Nuffel, Marceau Quatredeniers, Alexander Pirkl, Julia Zakel, Jean-Pierre Le Caër, et al.. Multimodal Imaging Mass Spectrometry to Identify Markers of Pulmonary Arterial Hypertension in Human Lung Tissue using MALDI-ToF, ToF-SIMS and Hybrid SIMS. Analytical Chemistry, 2020, 92 (17), pp.12079. ⟨10.1021/acs.analchem.0c02815⟩. ⟨hal-02908467⟩

Share

Metrics

Record views

165

Files downloads

21