Intracellular uranium distribution : Comparison of cryogenic fixation versus chemical fixation methods for SIMS analysis

D. Suhard (1) , C. Tessier (1) , Line Manens (2) , F. Rebière (3) , K. Tack (2) , Michelle Agarande (4) , Y. Guéguen (1)

1 IRSN/PSE-SANTE/SESANE/LRSI - Laboratoire de recherche en Radiochimie, Spéciation et Imagerie
2 IRSN/PSE-SANTE/SESANE/LRTOX - Laboratoire de radiotoxicologie et radiobiologie expérimentale
3 IRSN/PSE-SANTE/SESANE - Service de recherche sur les effets biologiques et Sanitaires des rayonnements ionisants
4 IRSN/PSE-ENV/SAME - Service d'analyses et de métrologie de l'environnement

Localization of uranium within cells is mandatory for the comprehension of its cellular mechanism of toxicity. Secondary Ion Mass Spectrometry (SIMS) has recently shown its interest to detect and localize uranium at very low levels within the cells. This technique requires a specific sample preparation similar to the one used for Transmission Electronic Microscopy, achieved by implementing different chemical treatments to preserve as much as possible the living configuration uranium distribution into the observed sample. This study aims to compare the bioaccumulation sites of uranium within liver or kidney cells after chemical fixation and cryomethods preparations of the samples SIMS analysis of theses samples show the localization of uranium soluble forms in the cell cytoplasm and nucleus with a more homogenous distribution when using cryopreparation probably due to the diffusible portion of uranium inside the cytoplasm. © 2018 The Authors. Microscopy Research and Technique Published by Wiley Periodicals, Inc.

Domaines: Sciences du Vivant [q-bio]

D. Suhard, C. Tessier, Line Manens, F. Rebière, K. Tack, et al.. Intracellular uranium distribution : Comparison of cryogenic fixation versus chemical fixation methods for SIMS analysis. Microscopy Research and Technique, 2018, 81 (8), pp.855-864. ⟨10.1002/jemt.23047⟩. ⟨hal-02874521⟩