Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping

Luro François (1) , Gilles Costantino (1) , Javier Terol (2) , Thierry Allario (3) , Xavier Argout (4) , Patrick Wincker (5) , Manuel Talón (4) , Patrick Ollitrault (6) , Raphaël Morillon (3)

1 Station de recherches agronomiques de San Giuliano
2 Centro de Genómica - Centre de Genòmica [IVIA]
3 UPR Multiplication végétative - Amélioration génétique d'espèces à multiplication végétative
4 Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement
5 CNRS - Centre National de la Recherche Scientifique
6 MONTPELLIER

During the last decade, numerous microsatellite markers were developed for genotyping and to identify closely related plant genotypes. In citrus, previously developed microsatellite markers were arisen from genomic libraries and more often located in non coding DNA sequences. To optimize the use of these EST-SSRs as genetic markers in genome mapping programs and citrus systematic analysis, we have investigated their polymorphism related to the type (di or trinucleotide) or their position in the coding sequences.

Mots clés: MICROSATELLITE MARKERS; MOLECULAR MARKERS; GENOME ANALYSIS; LINKAGE MAP; BREAD WHEAT; DIVERSITY; GERMPLASM; BARLEY; CLEMENTINIER; EST-SSR MARKERS; CITRUS

Mots clés: PONCIRUS-TRIFOLIATA; LOCI

Domaines: Génétique

Luro François, Gilles Costantino, Javier Terol, Thierry Allario, Xavier Argout, et al.. Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping. BMC Genomics, 2008, 9, pp.287. ⟨10.1186/1471-2164-9-287⟩. ⟨hal-02661072⟩