TREC-IN: gene knock-in genetic tool for genomes cloned in yeast.

Suchismita Chandran (1) , Vladimir N Noskov (1) , Thomas H Segall-Shapiro (1) , Li Ma (1) , Caitlin Whiteis (1) , Carole Lartigue (2) , Joerg Jores (3) , Sanjay Vashee (1) , Ray-Yuan Chuang (1)

1 JCVI - J. Craig Venter Institute [La Jolla, USA]
2 BFP - Biologie du fruit et pathologie
3 ILRI - International Livestock Research Institute [CGIAR, Nairobi]

Although we have demonstrated the feasibility of TREC-IN in gene complementation and genome minimization studies in Mmc, the applicability of TREC-IN ranges widely. This method proves to be a valuable genetic tool that can be extended for genomic engineering in other genetically intractable organisms, where it may be implemented in elucidating specific metabolic pathways and in rationale vaccine design.

Mots clés: mycoplasma; genomes; gene knock-in; yeast; autonomously replicating sequence; genome minimization

Mots clés: TREC

Domaines: Microbiologie et Parasitologie; Médecine vétérinaire et santé animale

Suchismita Chandran, Vladimir N Noskov, Thomas H Segall-Shapiro, Li Ma, Caitlin Whiteis, et al.. TREC-IN: gene knock-in genetic tool for genomes cloned in yeast.. BMC Genomics, 2014, 15 (1), pp.1180. ⟨10.1186/1471-2164-15-1180⟩. ⟨hal-02641317⟩