Current impact and future directions of high throughput sequencing in plant virus diagnostics

Sebastien Massart (1) , Antonio Olmos (2) , Haissam Jijakli (1) , Thierry T. Candresse (3)

1 Agro-Bio Tech
2 IVIA - Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research
3 BFP - Biologie du fruit et pathologie

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of high-throughput sequencing (also called Next Generation Sequencing - NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed.

Keywords: virus detection; virus diagnostic; high throughput sequencing; next generation sequencing; trends; plant virus; plant certification

Domains: Other [q-bio.OT]

Sebastien Massart, Antonio Olmos, Haissam Jijakli, Thierry T. Candresse. Current impact and future directions of high throughput sequencing in plant virus diagnostics. Virus Research, 2014, 188, pp.90-96. ⟨10.1016/j.virusres.2014.03.029⟩. ⟨hal-02635445⟩