Trichostatin A treatment of cloned mouse embryos improves constitutive heterochromatin remodeling as well as developmental potential to term

Walid W. Maalouf (1) , Zichuan Z. Liu (1) , Vincent V. Brochard (1) , Jean-Paul Renard (2) , Pascale Debey (3) , Nathalie Beaujean (1) , Daniele Zink

1 BDR - Biologie du développement et reproduction
2 Hôpital d'instruction des armées du Val de Grâce
3 Dipartimento di Biologia e Genetica per le Scienze Mediche [Milano, Italy]

Genome reprogramming in early mouse embryos is associated with nuclear reorganization and particular features such as the peculiar distribution of centromeric and pericentric heterochromatin during the first developmental stage. This zygote-specific heterochromatin organization could be observed both in maternal and paternal pronuclei after natural fertilization as well as in embryonic stem (ES) cell nuclei after nuclear transfer suggesting that this particular type of nuclear organization was essential for embryonic reprogramming and subsequent development.

Mots clés: EMBRYONIC DEVELOPEMENT; EMBRYO; HETEROCHROMATIN; MICE

Mots clés: NUCLEAR TRANSFER TECHNIQUES; DEVLOPPEMENT

Domaines: Biologie du développement; Biologie de la reproduction; Sciences du Vivant [q-bio]

Walid W. Maalouf, Zichuan Z. Liu, Vincent V. Brochard, Jean-Paul Renard, Pascale Debey, et al.. Trichostatin A treatment of cloned mouse embryos improves constitutive heterochromatin remodeling as well as developmental potential to term. BMC Developmental Biology, 2009, 9 (1), pp.11. ⟨10.1186/1471-213X-9-11⟩. ⟨hal-02610646⟩