Nanoceria Can Act as the Cues for Angiogenesis in Tissue-Engineering Scaffolds: Toward Next-Generation in Situ Tissue Engineering
Résumé
Next generation tissue engineering, exploits the body's own regenerating capacity by providing an optimum niche by means of a scaffold for the migration and subsequent proliferation endogenous cells to the site of injury, enhance regeneration or healing and bypass laborious in vitro cell culture procedures. Such systems also required to have enough angiogenic capacity for the subsequent patency of implanted scaffolds. The exploitation of nano dimensional ceria's (nCeO 2) redox properties in in situ tissue engineering to promote cell angiogenesis is poorly understood. As a novel strategy, electrospun polycaprolactone (PCL) tissue engineering scaffolds containing nCeO 2 were fabricated and characterized for the morphological and physicomechanical properties. Further, in vitro and in vivo studies were performed to show the ability of nCeO 2 containing scaffolds to enhance cell adhesion and angiogenesis. These studies confirmed that nCeO 2 containing scaffolds supported cell adhesion and angiogenesis better than bare scaffolds. Gene expression studies had shown that angiogenesis related factors like HIF1α and VEGF were upregulated. These results suggest that incorporation of nCeO 2 plays important role in the novel scaffolds to promote cell adhesion and proliferation and can be used in diverse tissue engineering applications.