The architecture of monospecific microalgae biofilms
Résumé
Microalgae biofilms have been proposed as an efficient alternative to suspended cultures [1]. They offer
enhanced productivities and straightforward harvesting by simple scrapping. However, little is known about
their structure (spatial arrangement of cells, colonies and Extracellular PolymerS; EPS), which may
strongly impact bioprocess stability and productivity. For example, nutrient diffusion may decrease as a
function of the cell density or colonies size and light is attenuated and may become limiting for the deeper
cell layers in very thick biofilms [2]. In order to better understand the structure development in microalgae
biofilms, several microalgae strains were cultivated under static and dynamic conditions (the latter to
simulate conditions closer to reality) and their architecture characterized in situ using confocal laser
scanning microscopy.
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