Steady state emission of the fluorescent intermediate of Anabaena Sensory Rhodopsin as a function of light adaptation conditions

Alexandre Cheminal (1, 2) , Jérémie Léonard (1, 2) , Carlo Boldetti , K Jung , H Kandori , Stefan Haacke (1, 2)

1 IPCMS - Institut de Physique et Chimie des Matériaux de Strasbourg
2 IPCMS - Institut de Physique et Chimie des Matériaux de Strasbourg

Steady-state fluorescence measurements of the first excited state of the anabaena sensory rhodopsin (ASR), and Bacteriorhodopsin are reported for different light stabilization conditions, including the dark-adapted state. We determine the fluorescence spectra of both all-trans (AT), and 13-cis (13C) protonated Schiff base of retinal, and compare the effect of the proteins. Referenced against the fluorescence quantum yield of AT-bR (2.5 x 10(-4)) we find for AT-ASR, 13C-ASR, and 13C-bR the values of 3.3 x 10(-4), 0.8 x 10(-4), and 1.7 x 10(-4), respectively. Using reported excited state lifetimes, the radiative rates are deduced, and their differences discussed on the basis of a configuration-dependent oscillator strength.

Domaines: Chimie théorique et/ou physique

Alexandre Cheminal, Jérémie Léonard, Carlo Boldetti, K Jung, H Kandori, et al.. Steady state emission of the fluorescent intermediate of Anabaena Sensory Rhodopsin as a function of light adaptation conditions. Chemical Physics Letters, 2013, 587, pp.75-80. ⟨10.1016/j.cplett.2013.09.044⟩. ⟨hal-02435559⟩