Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial

Fabien Wauquier (1) , Elsa Mével (2) , Stéphanie Krisa (3) , Tristan Richard (4) , Josep Valls , Ruth Hornedo-Ortega , Henri Granel (1) , Line Boutin-Wittrant , Nelly Urban (5) , Juliette Berger (6) , Stéphane Descamps (7) , Jérôme Guicheux (2) , Claire Vinatier (8) , Laurent Beck (2) , Nathalie Meunier (9) , Adeline Blot (10) , Y. Wittrant (1)

1 UNH - Unité de Nutrition Humaine
2 RMeS - Regenerative Medicine and Skeleton
3 OENO - Unité de Recherche Oenologie [Villenave d'Ornon]
4 Modulation Artificielle des Genes Eucaryotes
5 Union Grap'Sud
6 CHELTER - Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias
7 Service d’Orthopédie Traumatologie [CHU Clermont-Ferrand]
8 UFR Odonto - Université de Nantes - UFR Odontologie
9 CRNH A - Centre de Recherche en Nutrition Humaine Auvergne [CHU Clermont-Ferrand]
10 CRNH d'Auvergne - Centre de Recherche en Nutrition Humaine d'Auvergne

Polyphenols are widely acknowledged for their health benefits, especially for the prevention of inflammatory and age-related diseases. We previously demonstrated that hydroxytyrosol (HT) and procyanidins (PCy), alone or in combination, drive preventive anti-osteoathritic effects in vivo. However, the lack of sufficient clinical evidences on the relationship between dietary phytochemicals and osteoarthritis remains. In this light, we investigated in humans the potential osteoarticular benefit of a grapeseed and olive extract (OPCO) characterized for its hydroxytyrosol (HT) and procyanidins (PCy) content. We first validated, in vitro, the anti-inflammatory and chondroprotective properties of the extract on primary cultured human articular chondrocytes stimulated by interleukin-1 beta (IL-1 beta). The sparing effect involved a molecular mechanism dependent on the nuclear transcription factor-kappa B (NF-kappa B) pathway. To confirm the clinical relevance of such a nutritional strategy, we designed an innovative clinical approach taking into account the metabolites that are formed during the digestion process and that appear in circulation after the ingestion of the OPCO extract. Blood samples from volunteers were collected following ingestion, absorption, and metabolization of the extract and then were processed and applied on human primary chondrocyte cultures. This original ex vivo methodology confirmed at a clinical level the chondroprotective properties previously observed in vitro and in vivo.

Keywords: osteoarthritis (OA); hydroxytyrosol; clinical trials

Keywords: cell biology; micronutrients; procyanidins

Domains: Rhumatology and musculoskeletal system; Geriatry and gerontology; Tissues and Organs [q-bio.TO]; Hematology

Fabien Wauquier, Elsa Mével, Stéphanie Krisa, Tristan Richard, Josep Valls, et al.. Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial. Nutrients, 2019, 11 (12), pp.3071. ⟨10.3390/nu11123071⟩. ⟨hal-02426362⟩