Isotropic-Resolution Tomographic Diffractive Microscopy

Bertrand Simon (1) , Matthieu Debailleul (2) , Mounir Houkal , Carole Ecoffet (3) , Jonathan Bailleul (2) , Joël Lambert (4) , Arnaud Spangenberg (3) , Hui Liu (5) , Olivier Soppera (3) , Olivier Haeberlé (2)

1 LP2N - Laboratoire Photonique, Numérique et Nanosciences
2 MIPS - Modélisation, Intelligence, Processus et Système
3 IS2M - Institut de Science des Matériaux de Mulhouse
4 Sans affiliation
5 USUHS - Uniformed Services University of the Health Sciences

Microscopy techniques based on recording of the optical field diffracted by the specimen, in amplitude and phase, like Digital Holographic Microscopy (DHM) have been a growing research topic in recent years. Tomographic acquisitions are possible if one is able to record information, while controlling variations of the specimen illumination. Classical approaches consider either illumination variation, simple to implement, but suffering fro the classical "missing cone" problem, or sample rotation, delivering images with quasi-isotropic, but lower resolution. We have developed an original-, combined tomographic diffractive microscope setup, making use of specimen rotation as well as illumination rotation, which is able to deliver images with an almost isotropic resolution better than 200 nm.

Mots clés: 3-D microscopy; Interference microscopy; Digital holography; Holographic interferometry

Domaines: Optique [physics.optics]; Autre; Polymères

Bertrand Simon, Matthieu Debailleul, Mounir Houkal, Carole Ecoffet, Jonathan Bailleul, et al.. Isotropic-Resolution Tomographic Diffractive Microscopy. 2018 20th International Conference on Transparent Optical Networks (ICTON), Jul 2018, Bucharest, Romania. pp.1-4, ⟨10.1109/ICTON.2018.8473709⟩. ⟨hal-02388802⟩