Variation in Wolbachia cidB gene, but not cidA , is associated with cytoplasmic incompatibility mod phenotype diversity in Culex pipiens

Manon Bonneau (1, 2) , Beniamino Caputo (3) , Aude Ligier (1) , Rudy Caparros (4) , Sandra Unal (1) , Marco Perriat‐sanguinet (1) , Daniele Arnoldi (5) , Mathieu Sicard (1) , Mylène Weill (1)

1 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier
2 IGC - Instituto Gulbenkian de Ciência [Oeiras]
3 Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti
4 Université de Liège
5 Centro Ricerca e Innovazione - Research and Innovation Centre [FEM - Italie]

Endosymbiotic Wolbachia bacteria are, to date, considered the most widespread symbionts in arthropods and are the cornerstone of major biological control strategies. Such a high prevalence is based on the ability of Wolbachia to manipulate their hosts' reproduction. One manipulation called cytoplasmic incompatibility (CI) is based on the death of the embryos generated by crosses between infected males and uninfected females or between individuals infected with incompatible Wolbachia strains. CI can be seen as a modification-rescue system (or mod-resc) in which paternal Wolbachia produce mod factors, inducing embryonic defects, unless the maternal Wolbachia produce compatible resc factors. Transgenic experiments in Drosophila melanogaster and Saccharomyces cerevisiae converged towards a model where the cidB Wolbachia gene is involved in the mod function while cidA is involved in the resc function. However, as cidA expression in Drosophila males was required to observe CI, it has been proposed that cidA could be involved in both resc and mod functions. A recent correlative study in natural Culex pipiens mosquito populations has revealed an association between specific cidA and cidB variations and changes in mod phenotype, also suggesting a role for both these genes in mod diversity. Here, by studying cidA and cidB genomic repertoires of individuals from newly sampled natural C. pipiens populations harbouring wPipIV strains from North Italy, we reinforce the link between cidB variation and mod phenotype variation fostering the involvement of cidB in the mod phenotype diversity. However, no association between any cidA variants or combination of cidA variants and mod phenotype variation was observed. Taken together our results in natural C. pipiens populations do not support the involvement of cidA in mod phenotype variation.

Mots clés: Culex pipiens; Wolbachia; cidA; cidB; cytoplasmic incompatibility

Domaines: Interactions entre organismes; Zoologie des invertébrés; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Manon Bonneau, Beniamino Caputo, Aude Ligier, Rudy Caparros, Sandra Unal, et al.. Variation in Wolbachia cidB gene, but not cidA , is associated with cytoplasmic incompatibility mod phenotype diversity in Culex pipiens. Molecular Ecology, 2019, 28 (21), pp.4725-4736. ⟨10.1111/mec.15252⟩. ⟨hal-02386859⟩