Electrochemiluminescence Imaging for Bioanalysis

Jingjing Zhang (1) , Stéphane Arbault (2) , Neso Sojic (2) , Dechen Jiang

1 CAU - China Agricultural University
2 ISM - Institut des Sciences Moléculaires

Electrochemiluminescence (ECL) is a widely used analytical technique with the advantages of high sensitivity and low background signal. The recently fast development of electrochemical materials, luminophores and optical elements significantly increase the ECL signals, and thus, the ECL imaging with enhanced spatial and temporal resolutions is realized. Currently, ECL imaging has been successfully applied in high throughput bioanalysis and to visualize the distribution of molecules at single cells. Compared with other optical bioassays, no optical excitation is involved in imaging approach so that it avoids background signal from the illumination and increases the detection sensitivity. This review highlights some of the most exciting developments in this field, including the mechanisms, the electrode designs and the applications of ECL imaging in bioanalysis and at single cells and particles.

Mots clés: Electrochemiluminescence imaging; spatial/temporal resolutions; multiplex bioassay; single cells/particles; co-reactant pathway; bipolar electrochemistry

Domaines: Chimie analytique; Chimie théorique et/ou physique

Jingjing Zhang, Stéphane Arbault, Neso Sojic, Dechen Jiang. Electrochemiluminescence Imaging for Bioanalysis. Annual Review of Analytical Chemistry, 2019, 12 (1), pp.275-295. ⟨10.1146/annurev-anchem-061318-115226⟩. ⟨hal-02374932⟩