Efficient Antifouling Surface for Quantitative Surface Plasmon Resonance Based Biosensor Analysis

C. Nogues (1) , H Leh , J. Lautru (2) , O. Delelis (3) , Malcolm Buckle (3)

1 ISCR - Institut des Sciences Chimiques de Rennes
2 L2ME - Etude de la Matière en Mode Environnemental
3 LBPA - Laboratoire de Biologie et de Pharmacologie Appliquée

Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.

Domaines: Sciences du Vivant [q-bio]; Biotechnologies

C. Nogues, H Leh, J. Lautru, O. Delelis, Malcolm Buckle. Efficient Antifouling Surface for Quantitative Surface Plasmon Resonance Based Biosensor Analysis. PLoS ONE, 2012, 7, ⟨10.1371/journal.pone.0044287⟩. ⟨hal-02368517⟩