SPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune Hepatitis

Abstract : Background : Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH). Aim : To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2/B1 and serafrom patients and healthy controls. Methods : Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonanceImagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactionsinvolving the immobilised peptides were followed in real time and dissociation rate constants koff for each interactionwere calculated. Results : Several significant interactions were observed: i) high stability (lower koff values) between P55-70 and the AIHsera compared to controls (p= 0.003); ii) lower stability (higher koff values) between P118-133 and P262-277 and SLE sera,P145-160 and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and koff values observedafter the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serumindicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1and P55-70 and also between controls and the peptides studied. Conclusions : These results indicate that P55-70 of hnRNP B1 is a potential biomarker for AIH in immunological testsand suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmunedisorders and involved in antigen-antibody stability.
Complete list of metadatas

Cited literature [28 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02368493
Contributor : Malcolm Buckle <>
Submitted on : Monday, November 18, 2019 - 3:30:34 PM
Last modification on : Friday, December 6, 2019 - 10:26:56 AM

File

PlosOne2013_BeleokenE_BallotE_...
Publisher files allowed on an open archive

Identifiers

  • HAL Id : hal-02368493, version 1

Citation

Elvire Beleoken, Hervé Leh, Armelle Arnoux, Béatrice Ducot, Claude Nogues, et al.. SPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune Hepatitis. PLoS ONE, Public Library of Science, 2013. ⟨hal-02368493⟩

Share

Metrics

Record views

51

Files downloads

14