Subcellular distribution of 2′,5′-oligoadenylate synthetase in differentiating Friend leukemia cells

Nadir Mechti (1) , Elisabetta Affabris , Gianna Romeo , Bernard Lebleu (2)

1 IRIM - Institut de Recherche en Infectiologie de Montpellier
2 DIMNP - Dynamique des interactions membranaires normales et pathologiques

The occurrence of distinct (2'-5')(A)n-synthetase activities has recently been documented in cytoplasmic and nuclear extracts of several interferon (IFN)-treated cell lines. Since a role has been proposed for (2'-5')(A)n synthetase in the control of cell growth and differentiation, we examined the subcellular distribution of (2'-5')(A)n-synthetase activity both in IFN-treated undifferentiated Friend leukemia cells (FLCs) and during dimethyl-sulfoxide (DMSO)-induced erythroid differentiation of FLCs. Both the nuclear and cytoplasmic (2'-5')(A)n activities were modulated to the same extent by IFNs and DMSO. No evidence for a causal relationship between enzyme activation and FLC differentiation was found.

Domaines: Sciences du Vivant [q-bio]

Nadir Mechti, Elisabetta Affabris, Gianna Romeo, Bernard Lebleu. Subcellular distribution of 2′,5′-oligoadenylate synthetase in differentiating Friend leukemia cells. Differentiation, 1985, 29 (2), pp.136-139. ⟨10.1111/j.1432-0436.1985.tb00306.x⟩. ⟨hal-02361150⟩