Establishment of the antiviral state in alpha, beta-interferon-resistant Friend cells treated with gamma-interferon. Induction of 67-kilodalton protein kinase activity in absence of detectable 2-5A synthetase.

G. Romeo (1) , E Affabris , M Federico , Nadir Mechti (2) , E. Coccia (3) , C Jemma , G. Rossi (4)

1 IACR - International Agency for Cancer Research
2 IRIM - Institut de Recherche en Infectiologie de Montpellier
3 OCA - Observatoire de la Côte d'Azur
4 Sejong University

Treatment with murine gamma-interferon (IFN) preparations of variant sublines of Friend leukemia cells resistant to the alpha, beta IFN-induced antiviral state (Affabris, E., Jemma, C., and Rossi, G.B. (1982) Virology 120, 441-452; Affabris, E., Romeo, G., Belardelli, F., Jemma, C., Mechti, N., Gresser, I., and Rossi, G. B. (1983) Virology 125, 508-512) results in the establishment of a bona fide antiviral state. In fact, gamma IFN preparations are able to induce a dose-dependent reduction of endogenous virus release and of vesicular stomatitis or encephalomyocarditis viruses yields (up to 1.5 log). Under these experimental conditions, no inducible 2-5A synthetase activity is detectable in cell extracts. The 67-kDa protein kinase, uninducible by treatment with alpha, beta IFN (up to 13,000 units/ml), is instead induced upon treatment with gamma IFN at a similar rate of activity as in wild-type Friend leukemia cells, both when assayed in solution and after immobilization on poly(rI) X poly(rC)-agarose.

Domaines: Sciences du Vivant [q-bio]

G. Romeo, E Affabris, M Federico, Nadir Mechti, E. Coccia, et al.. Establishment of the antiviral state in alpha, beta-interferon-resistant Friend cells treated with gamma-interferon. Induction of 67-kilodalton protein kinase activity in absence of detectable 2-5A synthetase.. Journal of Biological Chemistry, 1985, 260 (6), pp.3833-8. ⟨10.1016/S0021-9258(19)83699-2⟩. ⟨hal-02361139⟩