Nuclear location of synthetic oligonucleotides microinjected somatic cells: its implication in an antisense strategy.

Nadir Mechti (1) , J Leonetti , J Clarenc , G Degols , B. Lebleu (2)

1 IRIM - Institut de Recherche en Infectiologie de Montpellier
2 IGMM - Institut de Génétique Moléculaire de Montpellier

Synthetic oligonucleotides are widely used to modulate gene expression. However their development as genetic tools and as molecule of therapeutic interest is restricted by the poor knowledge of their mechanism of action and of their uptake by cells. We recently found that microinjected oligonucleotides accumulates in the nucleus of the cells. These observations are described and their implications are discussed.

Domaines: Sciences du Vivant [q-bio]

Nadir Mechti, J Leonetti, J Clarenc, G Degols, B. Lebleu. Nuclear location of synthetic oligonucleotides microinjected somatic cells: its implication in an antisense strategy.. Nucleic Acids Symposium Series, 1991, 24, pp.147-50. ⟨hal-02361101⟩