Crystallization and preliminary X-ray crystallographic analysis of Human Geminin Coiled-coil domain

Michael Thépaut (1) , Christian Dumas , Bernard Calas , Marie-Paule Strub , André Padilla

1 CBS - Centre de Biochimie Structurale [Montpellier]

Initially discovered in Xenopus laevis, Geminin is a DNA replication initiation inhibitor found in higher eukaryotes. The coiled-coil domain of Human Geminin (termed GemH-37) has been crystallized by the vapor-diffusion sitting-drop method. A complete 1.74 Å data set has been collected on a single orthorhombic crystal with unit cell parameters a = 25.25, b = 44.35, c = 68.58 Å. Successful molecular replacement shows that GemH-37 is a dimeric parallel coiled-coil. Structural analysis is now in progress. D

Mots clés: Geminin; Crystallization; Replication initiation; Coiled coil

Domaines: Biologie structurale [q-bio.BM]

Michael Thépaut, Christian Dumas, Bernard Calas, Marie-Paule Strub, André Padilla. Crystallization and preliminary X-ray crystallographic analysis of Human Geminin Coiled-coil domain. Biochimica et Biophysica Acta (BBA) - Enzymology, 2002, ⟨10.1016/S1570-9639(02)00391-6⟩. ⟨hal-02359548⟩