Time of response to hormonal treatment but not the type of a spawning agent affects the reproductive effectiveness in domesticated pikeperch, Sander lucioperca

Daniel Żarski (1, 2) , Pascal Fontaine (1) , Jennifer Roche (3) , Maud Alix (4) , Miroslav Blecha (4) , Coralie Broquard (1) , Jarosław Król (5) , Sylvain Milla (1)

1 URAFPA - Unité de Recherches Animal et Fonctionnalités des Produits Animaux
2 Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research
3 AFMB - Architecture et fonction des macromolécules biologiques
4 Asialor
5 University of Warmia and Mazury [Olsztyn]

Improving reproductive protocols is one crucial step towards aquaculture expansion of pikeperch (Sander lucioperca), which is still characterised by variable and/or low spawning effectiveness. One of the main challenges is to synchronise ovulation at a precisely planned time with a consistently satisfactory reproductive outcome. To this end, the present study examined the effect of different spawning agents (human chorionic gonadotropin [hCG] and salmon gonadotropin-releasing hormone analogue [sGnRHa]) with different doses and application modes (including double injection). The study covered three consecutive spawning seasons, which corresponded to three distinct experiments, where domesticated pikeperch broodstock, commercially grown in a recirculating aquaculture system, was used. In the first year of the study, the effect of different doses of sGnRHa (10, 25 and 50 μg kg-1) and hCG (250, 500 and 1000 IU kg-1) on the reproductive performance of the domesticated broodstock was evaluated. The results were also compared with literature data for wild fish. During the second and third years, typical indicators of spawning performance (ovulation rate, latency time and egg quality) were followed when a double sGnRHa injection was compared to a single 50 µg kg-1 or 500 IU kg-1 injection of sGnRHa or hCG, respectively; the best results were

Domaines: Zoologie des vertébrés

Daniel Żarski, Pascal Fontaine, Jennifer Roche, Maud Alix, Miroslav Blecha, et al.. Time of response to hormonal treatment but not the type of a spawning agent affects the reproductive effectiveness in domesticated pikeperch, Sander lucioperca. Aquaculture, 2019, 503, pp.527-536. ⟨10.1016/j.aquaculture.2019.01.042⟩. ⟨hal-02359177⟩