Drug specific resistance to oxaliplatin is associated with apoptosis defect in a cellular model of colon carcinoma

Abstract : To investigate acquired resistance to oxaliplatin, we selected two resistant clones from the HCT116 cell line. We found that the resistant phenotype was associated with resistance to oxaliplatin-induced apoptosis as demonstrated by FACS analysis and by Western blotting of caspase 3 activation. In addition, the resistant phenotype showed a concomitant resistance to lonidamine and arsenic trioxide which are inducers of mitochondrial apoptosis. Furthermore, a complete loss of Bax expression due to a frameshift mutation was observed in the most resistant clone. Taken together, these findings suggest that altered mitochondrial-mediated apoptosis could play a role in oxaliplatin resistance.
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Submitted on : Thursday, November 7, 2019 - 3:55:00 PM
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Isabelle Gourdier, Maguy del Rio, Laure Crabbe, Laurent Candeil, Virginie Copois, et al.. Drug specific resistance to oxaliplatin is associated with apoptosis defect in a cellular model of colon carcinoma. FEBS Letters, Wiley, 2002, 529 (2-3), pp.232-236. ⟨10.1016/s0014-5793(02)03347-1⟩. ⟨hal-02354155⟩

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