Detection of IgG directed against a recombinant form of Epstein-Barr virus BALF0/1 protein in patients with nasopharyngeal carcinoma

Abstract : BALF0/1 is a putative Epstein-Barr virus (EBV) protein that has been described as a modulator of apoptosis. So far, the lack of specific immunological reagents impaired the detection of native BALF0/1 in EBV-infected cells. This study describes the expression and purification of a truncated form of BALF0/1 (tBALF0) using a heterologous bacterial expression system. tBALF0 was further used as an antigen in an indirect Enzyme-linked Immunosorbent Assay (ELISA) that unraveled the presence of low titer IgGs to BALF0/1 during primary (10.0%) and past (13.3%) EBV infection. Conversely high-titer IgGs to BALF0/1 were detected in 33.3% of nasopharyngeal carcinoma (NPC) patients suggesting that BALF0/1 and/or humoral response against it may contribute to NPC pathogenesis.
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https://hal.archives-ouvertes.fr/hal-02350124
Contributor : Stéphane Lemaire <>
Submitted on : Tuesday, November 5, 2019 - 11:00:09 PM
Last modification on : Saturday, November 9, 2019 - 2:08:52 AM

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Zhouwulin Shao, Chloé Borde, Christophe Marchand, Stéphane Lemaire, Pierre Busson, et al.. Detection of IgG directed against a recombinant form of Epstein-Barr virus BALF0/1 protein in patients with nasopharyngeal carcinoma. Protein Expression and Purification, Elsevier, 2019, 162, pp.44-50. ⟨10.1016/j.pep.2019.05.010⟩. ⟨hal-02350124⟩

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