Modulatory effects of sesame oil and ascorbic acid on abamectin-induced oxidative stress in rat liver and brain tissues

Abstract : The present work was designed to assess the modulatory effects of sesame oil (SO) and ascorbic acid (AA) on abamectin (ABM)-induced oxidative stress and altered gene expression of hepatic cytochrome P450 2E1 (CYP-2E1), p38 MAPK, and caspase-3 and cerebral P-glycoprotein (Abcb1a receptor). Male rats were distributed into five groups (6 rats/group), receiving distilled water, ABM 2 mg/kg bwt 1/5 LD50 orally for 5 days, ABM+AA 100 mg/kg bwt orally, ABM+SO 5 mL/kg bwt orally, or ABM+SO+AA at the aforementioned doses. Nineteen compounds were identified in the SO sample by GC-MS analysis, including tetradecane,2,6,10-trimethyl, octadecane, 1-hexadecanol,2-methyl, and octadecane,6-methyl. Abamectin significantly upregulated the hepatic CYP-2E1 expression with excess generation of oxidative radicals, as evident by the significant depletion of reduced glutathione and elevation of malondialdehyde concentration (p ≤ 0.05) in rat liver and brain tissues. Further, ABM significantly increased TNF-α concentration, the expression of caspase-3 and p38 MAPK in the liver, as well as p-glycoprotein and GABA-A receptor in the brain. These results were in line with the observed histopathological changes. Sesame oil and/or AA supplementation alleviated ABM-induced cell damage by modulating all tested parameters. In conclusion, ABM induces oxidative stress and increases the expression of CYP-2E1, caspase-3, and p38 MAPK in the liver, as well as P-gp and GABA-A receptor in the brain. These effects could be ameliorated by SO and AA, alone and in combination, probably due to their anti-oxidant, anti-apoptotic, and gene-regulating activities.
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https://hal.archives-ouvertes.fr/hal-02349580
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Submitted on : Tuesday, November 5, 2019 - 5:08:28 PM
Last modification on : Wednesday, November 6, 2019 - 1:26:38 AM

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Abeer M. Radi, Eman T. Mohammed, Abdelrahman Ibrahim Abushouk, Lotfi Aleya, Mohamed M. Abdel-Daim. Modulatory effects of sesame oil and ascorbic acid on abamectin-induced oxidative stress in rat liver and brain tissues. Science of the Total Environment, Elsevier, 2019, pp.134882. ⟨10.1016/j.scitotenv.2019.134882⟩. ⟨hal-02349580⟩

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