Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells

Lise Noack (1) , Přemysl Pejchar (2, 3) , Juraj Sekereš (3, 2) , Yvon Jaillais (1) , Martin Potocky (2, 3)

1 RDP - Reproduction et développement des plantes
2 IEB / CAS - Institute of Experimental Botany of the Czech Academy of Sciences
3 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]

Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein-phospholipid interactions enabled the design of genetically-encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.

Mots clés: transient expression; phosphoinositides; Nicotiana tabacum; Nicotiana benthamiana; phospholipid-binding domains; pollen tube; Microscopy

Domaines: Botanique; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Lise Noack, Přemysl Pejchar, Juraj Sekereš, Yvon Jaillais, Martin Potocky. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells. Fatima Cvrčková; Viktor Žárský. Plant Cell Morphogenesis. Methods and Protocols, 1992, Springer, pp.189-199, 2019, Methods in Molecular Biology, 978-1-4939-9468-7. ⟨10.1007/978-1-4939-9469-4_12⟩. ⟨hal-02348954⟩