P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes

Eléonore Bertin , Audrey Martinez (1) , Eric Boué-Grabot (1, 1)

1 IMN - Institut des Maladies Neurodégénératives [Bordeaux]

Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.

Mots clés: P2X; ATP-gated channels; Double-electrode patch clamp; Electrophysiology; Receptor function; Surface trafficking; Xenopus; Oocytes

Domaines: Neurobiologie

Eléonore Bertin, Audrey Martinez, Eric Boué-Grabot. P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes. Book title: Purinergic signaling Series Title: Methods in Molecular Biology Edited by Springer Nature, pp.243-259, 2020, ⟨10.1007/978-1-4939-9717-6_18⟩. ⟨hal-02347696⟩