Chloroplast lipid droplet type II NAD(P)H quinone oxidoreductase is essential for prenylquinone metabolism and vitamin K1 accumulation

Lucia Eugeni Piller , Céline Besagni , Brigitte Ksas (1) , Dominique Rumeau (1) , Claire Bréhélin (2) , Gaétan Glauser (3) , Felix Kessler (3) , Michel Havaux (1)

1 BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB)
2 LBM - Laboratoire de biogenèse membranaire
3 UNINE - Université de Neuchâtel = University of Neuchatel

Lipid droplets are ubiquitous cellular structures in eukaryotes and are required for lipid metabolism. Little is currently known about plant lipid droplets other than oil bodies. Here, we define dual roles for chloroplast lipid droplets (plastoglobules) in energy and prenylquinone metabolism. The prenylquinones-plastoquinone, plastochromanol-8, phylloquinone (vitamin K 1), and tocopherol (vitamin E)-are partly stored in plastoglobules. This work shows that NAD(P)H dehydrogenase C1 (NDC1) (At5g08740), a type II NAD(P)H quinone oxidoreductase, associates with plastoglobules. NDC1 reduces a plastoquinone analog in vitro and affects the overall redox state of the total plastoquinone pool in vivo by reducing the plastoquinone reservoir of plastoglobules. Finally, NDC1 is required for normal plastochromanol-8 accumulation and is essential for vitamin K 1 production. lipidomics | alternative electron flow | high light

Domaines: Sciences du Vivant [q-bio]; Biologie végétale

Lucia Eugeni Piller, Céline Besagni, Brigitte Ksas, Dominique Rumeau, Claire Bréhélin, et al.. Chloroplast lipid droplet type II NAD(P)H quinone oxidoreductase is essential for prenylquinone metabolism and vitamin K1 accumulation. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (34), pp.14354-14359. ⟨10.1073/pnas.1104790108⟩. ⟨hal-02347066⟩