Bioinspired crystallization, sensitized luminescence and cytocompatibility of citrate-functionalized Ca-substituted europium phosphate monohydrate nanophosphors

Abstract : Biocompatible nanosystems exhibiting long-lifetime (∼millisecond) luminescence features are particularly relevant in the field of bioimaging. In this study, citrate-functionalized calcium-doped europium phosphates nanophosphors of the rhabdophane type were prepared at different synthesis times by a bioinspired crystallization route, consisting in thermal decomplexing of Ca2+/Eu3+ /citrate/phosphate/carbonate solutions. The general formula of this material is CaαEu1-α(PO4)1-α(HPO4)α·nH2O, with α ranging from 0 to 0.58 and n ∼ 1. A thorough characterization of the nanoparticles has been carried out by XRD (including data processing with Topas 6.0), HR-TEM, TEM, FTIR, TG/DTA, ICP, dynamic light scattering (DLS), electrophoretic mobility, and fluorescence spectroscopy. Based on these results a crystallization mechanism involving the filling of cationic sites with Ca2+ions associated to a concomitant adjustment of the PO4/HPO4 ratio was proposed. Upon calcium doping, the aspect ratio of the nanoparticles as well as of the crystalline domains decreased and the relative luminescence intensity (R.L.I.) could be modulated. Neither the pH nor the ionic strength, nor the temperature (from 25 to 37 °C) affected significantly the R.L.I. of particles after resuspension in water, leading to rather steady luminescence features usable in a large domain of conditions. This new class of luminescent compounds has been proved to be fully cytocompatible relative to GTL-16 human carcinoma cells and showed an improved cytocompatibility as the Ca2+ content increased when contacted with the more sensitive m17. ASC murine mesenchymal stem cells. These biocompatible nanoparticles thus appear as promising new tailorable tools for biomedical applications as luminescent nanoprobes.
Document type :
Journal articles
Complete list of metadatas

Cited literature [68 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02328048
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Wednesday, October 23, 2019 - 9:51:49 AM
Last modification on : Friday, January 10, 2020 - 9:08:26 PM
Long-term archiving on: Friday, January 24, 2020 - 2:42:30 PM

File

Gomez-Morales_24534.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Jaime Gómez-Morales, Cristóbal Verdugo-Escamilla, Raquel Fernández-Penas, Carmen Maria Parra-Milla, Christophe Drouet, et al.. Bioinspired crystallization, sensitized luminescence and cytocompatibility of citrate-functionalized Ca-substituted europium phosphate monohydrate nanophosphors. Journal of Colloid and Interface Science, Elsevier, 2019, 538, pp.174-186. ⟨10.1016/j.jcis.2018.11.083⟩. ⟨hal-02328048⟩

Share

Metrics

Record views

22

Files downloads

49