S.E.C. OF STARCH MACROMOLECULES. THE ADVANTAGE OF «ON-LINE OPTICAL ROTATION MONITORING» IN REVEALING INTERMEDIATE MATERIAL AND POLYMODAL DISTRIBUTION IN AMYLOPECTIN
Résumé
Amongst the analytical methods used for the separation of macromolecules, SEC is one of the most widely-used.I, polysaccharide analysis, such as starch complexes, cross-linked dextrans Sepharose CL2B, designed for large macromolecular screening, has proved useful in the separation of amylose and amylopectin, detection being monitored by Iodine complexation analysis. Two major drawbacks of this detection method are the « tube by tube » analysis that implies lengthy manipulations, and the difference within both wavelength and iodine binding capacity for amylose or amylopectin. The existence in alpha- glucans of large polarised-light specific-rotation angles is another physicochemical characteristic, that can be used for the detection of starch macromolecules. The same physicochemical characteristic can be used to analyse low or non-iodine binding starch-like macromolecules, glycogen, phytoglycogen and many other polysaccharide families as well. In this paper we present the results of SEC separation of various starch-like material using on-line optical rotation monitoring. This method allowed us identify the presence of intermediate material and the existence of polymodal distribution in amylopectins.
