Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line

Céline Crauste (1) , Isabelle Lefebvre (1) , Michael Hovaneissian (1) , Jean Yves Puy (1) , Béatrice Roy (1) , Suzanne Peyrottes (1) , Sabine Cohen , Jérome Guitton (2) , Charles Dumontet (3) , Christian Périgaud (1)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 HCL - Hospices Civils de Lyon
3 UNICANCER/CRCL - Centre de Recherche en Cancérologie de Lyon

Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line a b s t r a c t A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge , a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 g mL −1 for araCTP and of 0.01 g mL −1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL.

Domaines: Chimie organique

Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Yves Puy, Béatrice Roy, et al.. Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line. Journal of Chromatography B Biomedical Sciences and Applications, 2009, 877 (14-15), pp.1417-1425. ⟨10.1016/j.jchromb.2009.02.071⟩. ⟨hal-02309584⟩