Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Abstract : We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.
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https://hal.archives-ouvertes.fr/hal-02282630
Contributor : Isabelle Frapart <>
Submitted on : Tuesday, September 10, 2019 - 10:46:45 AM
Last modification on : Wednesday, September 11, 2019 - 1:18:43 AM

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Béatrice Vallée, Michel Doudeau, Fabienne Godin, Hélène Bénédetti. Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein. Journal of visualized experiments : JoVE, JoVE, 2019, pp.e59820. ⟨10.3791/59820⟩. ⟨hal-02282630⟩

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