Exportin-5 mediates nuclear export of minihelix-containing RNAs

Abstract : The adenovirus VA1 RNA (VA1.), a 160-nucleotide (nt)- long RNA transcribed by RNA polymerase III, is efficiently exported from the nucleus to the cytoplasm of infected cells, where it antagonizes the interferon-induced antiviral defense system. We recently reported that nuclear export of VA1 is mediated by a cis-acting RNA export motif, called minihelix, that comprises a double-stranded stem (\textgreater 14 nt) with a base-paired 5' end and a 3-8-nt protruding 3' end. RNA export mediated by the minibelix motif is Ran-dependent, which indicates the involvement of a karyopherin-related factor (exportin) that remained to be determined. Here we show using microinjection in Xenopus laevis oocytes that VA1 is transported to the cytoplasm by exportin-5, a nuclear transport factor for double-stranded RNA binding proteins. Gel retardation assays revealed that exportin-5 directly interacts with VA1 RNA in a RanGTP-dependent manner. More generally, in vivo and in vitro competition experiments using various VA1-derived, but also artificial and cellular, RNAs lead to the conclusion that exportin-5 preferentially recognizes and transports minihelix motif-containing RNAs.
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Submitted on : Thursday, August 1, 2019 - 3:53:48 PM
Last modification on : Saturday, August 3, 2019 - 1:21:00 AM

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  • HAL Id : hal-02239917, version 1

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C. Gwizdek, B. Ossareh-Nazari, A. M. Brownawell, A. Doglio, E. Bertrand, et al.. Exportin-5 mediates nuclear export of minihelix-containing RNAs. Journal of Biological Chemistry, 2003, 278 (8), pp.5505--5508. ⟨hal-02239917⟩

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