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1 H -benzo[ d ]imidazole derivatives affect MmpL3 in Mycobacterium tuberculosis

Abstract : 1H-benzo[d]imidazole derivatives exhibit antitubercular activity in vitro at a nanomolar range of concentrations and are not toxic to human cells, but their mode of action remains unknown. Here, we showed that these compounds are active against intracellular Mycobacterium tuberculosis. To identify their target, we selected drug-resistant M. tuberculosis mutants and then used whole-genome sequenc-ing to unravel mutations in the essential mmpL3 gene, which encodes the integral membrane protein that catalyzes the export of trehalose monomycolate, a precursor of the mycobacterial outer membrane component trehalose dimycolate (TDM), as well as mycolic acids bound to arabinogalactan. The drug-resistant phenotype was also observed in the parental strain overexpressing the mmpL3 alleles carrying the mutations identified in the resistors. However, no cross-resistance was observed between 1H-benzo[d]imidazole derivatives and SQ109, another MmpL3 inhibitor, or other first-line antitubercular drugs. Metabolic labeling and quantitative thin-layer chromatography (TLC) analysis of radiolabeled lipids from M. tuberculosis cultures treated with the benzoimidazoles indicated an inhibition of trehalose dimycolate (TDM) synthesis, as well as reduced levels of mycolylated arabinogalactan, in agreement with the inhibition of MmpL3 activity. Overall, this study emphasizes the pronounced activity of 1H-benzo[d]imidazole derivatives in interfering with mycolic acid metabolism and their potential for therapeutic application in the fight against tuberculosis.
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Submitted on : Monday, November 16, 2020 - 1:36:40 PM
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Małgorzata Korycka-Machała, Albertus Viljoen, Jakub Pawełczyk, Paulina Borówka, Bożena Dziadek, et al.. 1 H -benzo[ d ]imidazole derivatives affect MmpL3 in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2019, 63, pp.441 - 460. ⟨10.1128/AAC.00441-19⟩. ⟨hal-02202451⟩

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