Quantification of lipids: model, reality, and compromise

Spiro Khoury (1) , Cécile Canlet (2) , Marlène Z. Lacroix (3) , Olivier Berdeaux (1) , Juliette Jouhet (4) , Justine Bertrand-Michel (5)

1 CSGA - Centre des Sciences du Goût et de l'Alimentation [Dijon]
2 E20 - Metatoul AXIOM
3 ENVT - Ecole Nationale Vétérinaire de Toulouse
4 LPCV - Physiologie cellulaire et végétale
5 UPS/Inserm U1297 - I2MC - Institut des Maladies Métaboliques et Casdiovasculaires

Lipids are key molecules in various biological processes, thus their quantification is a crucial point in a lot of studies and should be taken into account in lipidomics development. This family is complex and presents a very large diversity of structures, so analyzing and quantifying all this diversity is a real challenge. In this review, the different techniques to analyze lipids will be presented: from nuclear magnetic resonance (NMR) to mass spectrometry (with and without chromatography) including universal detectors. First of all, the state of the art of quantification, with the definitions of terms and protocol standardization, will be presented with quantitative lipidomics in mind, and then technical considerations and limitations of analytical chemistry's tools, such as NMR, mass spectrometry and universal detectors, will be discussed, particularly in terms of absolute quantification.

Mots clés: lipidomic; mass spectrometry; nuclear magnetic resonance; quantification; universal detector

Domaines: Biochimie, Biologie Moléculaire

Spiro Khoury, Cécile Canlet, Marlène Z. Lacroix, Olivier Berdeaux, Juliette Jouhet, et al.. Quantification of lipids: model, reality, and compromise. Biomolecules, 2018, 8 (4), pp.174. ⟨10.3390/biom8040174⟩. ⟨hal-02199860⟩