Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol–Ene or Thiol–Yne Coupling Chemistry

Maria-Jose Bañuls (1) , Pilar Jiménez-Meneses (1) , Albert Meyer (2) , Jean-Jacques Vasseur (2) , François Morvan (2) , Jorge Escorihuela (1) , Rosa Puchades (1) , Ángel Maquieira (1)

1 UPV - Universitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia
2 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]

Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.

Mots clés: CLICK CHEMISTRY; NUCLEIC-ACID; SILICON SURFACES; IMMOBILIZATION; FUNCTIONALIZATION; BIOSENSORS; PROTEINS; DEVICES

Domaines: Chimie analytique; Chimie organique; Chimie thérapeutique

Maria-Jose Bañuls, Pilar Jiménez-Meneses, Albert Meyer, Jean-Jacques Vasseur, François Morvan, et al.. Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol–Ene or Thiol–Yne Coupling Chemistry. Bioconjugate Chemistry, 2016, 28 (2), pp.496-506. ⟨10.1021/acs.bioconjchem.6b00624⟩. ⟨hal-02197214⟩