Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis

Xiahui Hu (1) , Ling Hu (1) , Juliette Jouhet (2) , Fabrice Rébeillé (2) , Eric Maréchal (2) , Hanhua Hu (3) , Yufang Pan (3) , Xiaoming Tan (4) , Zhuo Chen (5) , Lingjie You (1) , Hong Chen (1) , Fang Wei (1) , Yangmin Gong (1)

1 Oil Crops Research Institute of Chinese Academy of Agricultural Sciences
2 LPCV - Physiologie cellulaire et végétale
3 Institute of Hydrobiology - Chinese Academy of Sciences
4 Hubei Collaborative Innovation Center for Green Transformation of Bioresources. Key Laboratory of Industrial Biotechnology
5 Shandong Provincial Key Laboratory of Plant Stress

In photosynthetic oleaginous microalgae, acyl-CoA molecules are used as substrates for the biosynthesis of membrane glycerolipids, triacylglycerol (TAG) and other acylated molecules. Acyl-CoA can also be directed to beta-oxidative catabolism. They can be utilized by a number of lipid metabolic enzymes including endogenous thioesterases, which catalyze their hydrolysis to release free fatty acids. Acyl-CoA availability thus plays fundamental roles in determining the quantity and composition of membrane lipids and storage lipids.

Mots clés: Acyl-CoA; Acyl-CoA thioesterase; Fatty acids; Phaeodactylum tricornutum; TALEN; Triacylglycerols

Domaines: Biochimie, Biologie Moléculaire

Xiahui Hu, Ling Hu, Juliette Jouhet, Fabrice Rébeillé, Eric Maréchal, et al.. Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis. Biotechnology for Biofuels, 2018, 11 (1), pp.312. ⟨10.1186/s13068-018-1309-3⟩. ⟨hal-02196964⟩