Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex

M. Marin , F. Brockly , D. Noel (1) , M. Etienne-Julan , M. Biard-Piechaczyk , T. D. Hua , Z. J. Gu , Marc Piechaczyk (2, 3)

1 Service de Médecine Interne [CHU Rouen]
2 IBCP - Institut de biologie et chimie des protéines [Lyon]
3 IGMM - Institut de Génétique Moléculaire de Montpellier

B9.12.1 is a monoclonal antibody specific for a monomorphic determinant of human MHC class I molecules. It is currently used for cell typing and is useful for targeting infection of human cells by murine ecotropic retroviruses. We have cloned and expressed it in the form of a single-chain variable fragment (ScFv) that recognizes the same epitope as the parental antibody. Through genetic engineering, this ScFv may be used for developing new cell-typing probes and new retroviral targeting approaches.

Mots clés: Antibody Cloning; Immunoglobulin Genes; MHC Class I/immunology Histocompatibility Antigens Class I/*genetics/*immunology Humans Immunoglobulin Variable Region/biosynthesis/*genetics/isolation & purification Molecular Sequence Data; Molecular Epitopes/chemistry/*immunology Gene Expression/immunology *Genes; Amino Acid Sequence *Antibody Specificity Base Sequence Binding Sites

Domaines: Biochimie, Biologie Moléculaire

M. Marin, F. Brockly, D. Noel, M. Etienne-Julan, M. Biard-Piechaczyk, et al.. Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex. HYBRIDOMA, 1995, 14 (5), pp.443--51. ⟨10.1089/hyb.1995.14.443⟩. ⟨hal-02192672⟩