Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method

V. Ea , F. Court , T. Forne (1)

1 IGMM - Institut de Génétique Moléculaire de Montpellier

The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.

Mots clés: Chromatin dynamics and organization Chromosome conformation capture Quantitative PCR

Domaines: Biochimie, Biologie Moléculaire

V. Ea, F. Court, T. Forne. Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method. Methods in Molecular Biology, 2017, 1589, pp.75--88. ⟨10.1007/7651_2015_269⟩. ⟨hal-02187190⟩