Skip to Main content Skip to Navigation
Journal articles

Multiscale Structuring of the E. coli Chromosome by Nucleoid-Associated and Condensin Proteins

Abstract : As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture of the Escherichia coli genome, showing its partition into two structurally distinct entities by a complex and intertwined network of contacts: the replication terminus (ter) region and the rest of the chromosome. Outside of ter, the condensin MukBEF and the ubiquitous nucleoid-associated protein (NAP) HU promote DNA contacts in the megabase range. Within ter, the MatP protein prevents MukBEF activity, and contacts are restricted to ∼280 kb, creating a domain with distinct structural properties. We also show how other NAPs contribute to nucleoid organization, such as H-NS, which restricts short-range interactions. Combined, these results reveal the contributions of major evolutionarily conserved proteins in a bacterial chromosome organization.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02180787
Contributor : Marie-Claude Serre Connect in order to contact the contributor
Submitted on : Thursday, July 11, 2019 - 4:05:09 PM
Last modification on : Tuesday, November 23, 2021 - 2:38:07 PM

Links full text

Identifiers

Citation

Virginia S. Lioy, Axel Cournac, Martial Marbouty, Stéphane Duigou, Julien Mozziconacci, et al.. Multiscale Structuring of the E. coli Chromosome by Nucleoid-Associated and Condensin Proteins. Cell, Elsevier, 2018, 172 (4), pp.771--783.e18. ⟨10.1016/j.cell.2017.12.027⟩. ⟨hal-02180787⟩

Share

Metrics

Record views

227